Related Experiment Video

Updated: Dec 4, 2025

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
07:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

780

IL-18 and related function proteins associated with tuberculosis severity and screening for active TB among patients

Sebastian Wawrocki1, Michal Seweryn2, Grzegorz Kielnierowski3

  • 1Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.

Saudi Journal of Biological Sciences
|October 26, 2020
PubMed
Abstract

Insights

Distinguishing active pulmonary tuberculosis (TB) from community-acquired pneumonia (CAP) is challenging. Serum IP-10 and IL-18BP show promise as biomarkers for differentiating these respiratory conditions.

Area of Science:

  • Immunology
  • Infectious Diseases
  • Respiratory Medicine

Background:

  • Differentiating active pulmonary tuberculosis (TB) from community-acquired pneumonia (CAP) presents a significant diagnostic hurdle.
  • Accurate diagnosis is crucial for effective treatment and patient outcomes.

Purpose of the Study:

  • To evaluate serum and stimulated blood culture levels of IL-18, IFN-γ, IL-18BP, IL-37, and IP-10.
  • To determine the utility of these proteins in distinguishing between TB and CAP.

Main Methods:

  • Serum and Mycobacterium tuberculosis (M.tb) antigen-stimulated blood cultures were analyzed from 124 Polish adults.
  • Participants included patients with mild/moderate TB (M/MTB), advanced TB (ATB), and CAP.
  • Cytokine concentrations were quantified using enzyme-linked immunosorbent assay (ELISA).

Main Results:

  • Serum IP-10 and IL-18BP were the most specific and sensitive biomarkers for differentiating TB from CAP.
  • IP-10 demonstrated the highest area under the receiver operating characteristic curve (AUC).
  • Elevated serum IP-10 and IL-18BP levels were observed in both M/MTB and ATB groups, with IL-18BP elevation correlating with increased IL-18 in ATB patients. No significant differences were found in stimulated cultures.

Conclusions:

  • Combined analysis of serum IL-18BP and IP-10 may serve as an auxiliary diagnostic tool.
  • These biomarkers can aid in the differentiation of TB from CAP.
  • Further research may refine their role in clinical practice.
Keywords:
Community-acquired pneumoniaIL-18IL-18BPIP-10Tuberculosis

More Related Videos

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis09:57

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

Published on: April 5, 2017

9.0K
Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis07:32

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

806

Related Concept Videos

Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
309
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
669
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
574
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
1.0K
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
349
Pneumonia III: Complications and Assessment01:30

Pneumonia III: Complications and Assessment

Pneumonia poses the potential for numerous complications that warrant consideration. These complications include the following:
662