Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Liver Histology

Liver Histology

The microscopic anatomy of the liver is a complex and intricate system that comprises numerous structural units known as liver lobules, each of which is comparable in size to a sesame seed. These hexagonal structures consist of plates of liver cells or hepatocytes, which are characterized by their versatility and abundance of cellular apparatus like rough and smooth ER, Golgi apparatus, peroxisomes, and mitochondria.
Hepatocytes perform a variety of essential functions. They secrete...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Overcoming CXCR4-Mediated T-Cell Exclusion Potentiates Antitumor Cytotoxicity in Fibrolamellar Carcinoma.

Gastroenterology·2026

Same author

Ontogeny and functional potential of founding dendritic cells in the developing lung.

bioRxiv : the preprint server for biology·2025

Same author

Intratumoral Three-Cell-Type Clusters Are a Conserved Feature of Endogenous Antitumor Immunity.

Cancer immunology research·2025

Same author

Antigen specificities and proviral integration sites differ in HIV-infected cells by timing of antiretroviral treatment initiation.

The Journal of clinical investigation·2024

Same author

Optimizing a linear 'Doggybone' DNA vaccine for influenza virus through the incorporation of DNA targeting sequences and neuraminidase antigen.

Discovery immunology·2024

Same author

Pharmacologic enhancement of retromer rescues endosomal pathology induced by defects in the Alzheimer's gene SORL1.

Stem cell reports·2023

Search research articles

Related Experiment Video

Updated: Dec 4, 2025

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

Published on: May 24, 2011

Human Liver Macrophage Subsets Defined by CD32.

Xia Wu¹, Nicole Hollingshead¹, Jessica Roberto¹

¹Departments of Pathology, University of Washington Medical Center, Seattle, WA, United States.

Frontiers in Immunology

|October 26, 2020

Summary

This summary is machine-generated.

Researchers identified two distinct subsets of human liver myeloid cells, differing in surface markers and gene expression. These subsets suggest unique roles in T cell tolerance and may challenge current definitions of liver macrophages and dendritic cells.

Keywords:

CD32 Kupffer cell human liver myeloid

More Related Videos

Selective Harvesting of Marginating-hepatic Leukocytes

Selective Harvesting of Marginating-hepatic Leukocytes

Published on: July 21, 2016

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

Related Experiment Videos

Last Updated: Dec 4, 2025

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

A Simple and Efficient Method to Isolate Macrophages from Mixed Primary Cultures of Adult Liver Cells

Published on: May 24, 2011

Selective Harvesting of Marginating-hepatic Leukocytes

Selective Harvesting of Marginating-hepatic Leukocytes

Published on: July 21, 2016

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Published on: November 16, 2021

Area of Science:

Immunology
Cell Biology
Hepatology

Background:

Human liver myeloid cells, including Kupffer cells (liver macrophages), are not fully characterized.
Previous single-cell RNA sequencing studies suggest heterogeneity within liver macrophage populations.

Purpose of the Study:

To define and characterize distinct subsets of macrophage-like cells in human liver.
To investigate the potential functional differences between these subsets, particularly in T cell interactions.

Main Methods:

Analysis of dissociated human liver tissue using CD11b and CD68 markers.
Fluorescence-activated cell sorting (FACS) based on CD32 expression to isolate subsets.
Gene expression analysis (qRT-PCR) to identify subset characteristics and functions.

Main Results:

Two distinct subsets of CD11b+CD68+ myeloid cells were identified in the human liver.
These subsets exhibited differential expression of surface markers and genes related to T cell activation and immunosuppression.
One subset expressed CD1C and CD11c, markers typically associated with classical dendritic cells.

Conclusions:

The human liver contains at least two distinct populations of myeloid cells with macrophage characteristics.
These subsets may play different roles in regulating T cell responses and maintaining immune tolerance.
Current criteria for distinguishing macrophages from dendritic cells in the human liver may require re-evaluation.