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A Survey of Regulatory Interactions Among RNA Binding Proteins and MicroRNAs in Cancer

Ying Liu1,2, Chu Pan1,2, Dehan Kong3

  • 1College of Computer Science and Electronic Engineering, Hunan University, Changsha, China.

Frontiers in Genetics
|October 26, 2020
PubMed

Insights

RNA binding proteins (RBPs) and microRNAs interact in cancer, influencing gene expression. This study analyzed their binding sites and expression data to reveal widespread regulatory interactions affecting disease.

Area of Science:

  • Genomics and Proteomics
  • Molecular Biology
  • Cancer Research

Background:

  • Advances in genomics and proteomics yield extensive trans-regulatory data, including RNA binding proteins (RBPs) and microRNAs.
  • Many trans-regulators target the 3' untranslated region (UTR) of mRNA, suggesting potential interactions like competitive or cooperative effects.

Purpose of the Study:

  • To investigate interactions between RBPs and microRNAs.
  • To correlate RBP and microRNA binding data with mRNA expression in cancer.

Main Methods:

  • Compiled available RBP and microRNA binding sites.
  • Mapped binding sites to mRNA transcripts.
  • Correlated binding data with mRNA expression data from The Cancer Genome Atlas (TCGA) using regression analysis.

Main Results:

  • Observed significant interaction effects between RBPs and microRNAs in most cancer expression datasets.
  • Categorized RBP-microRNA pairs into overlapping, neighboring, and independent target site scenarios.
  • Demonstrated widespread interactions influencing mRNA expression profiles.

Conclusions:

  • Widespread interactions between RBPs and microRNAs are evident in cancer.
  • These interactions have significant implications for understanding cancer and disease development.
  • The findings highlight a complex regulatory network involving RBPs and microRNAs in oncogenesis.
Keywords:
RNA binding proteinscancergene expressiongene regulationmicroRNAsregression analysis

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