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Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

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Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
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Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

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The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Hedgehog Signaling Pathway02:33

Hedgehog Signaling Pathway

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The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
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Notch Signaling Pathway03:14

Notch Signaling Pathway

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The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Related Experiment Video

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Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling
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Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development.

De-Li Shi1

  • 1Developmental Biology Laboratory, CNRS-UMR 7622, IBPS, Sorbonne University, Paris, France.

Frontiers in Cell and Developmental Biology
|October 26, 2020
PubMed
Summary

Dishevelled proteins regulate Wnt signaling during early vertebrate development. Understanding their complex roles and pathway specificity is crucial for developmental biology and disease research.

Keywords:
DishevelledWnt signalingWnt/PCPWnt/ß-cateninXenopusconvergence and extensionmousezebrafish

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Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
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Related Experiment Videos

Last Updated: Dec 4, 2025

Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling
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Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Signaling

Background:

  • Dishevelled (DVL) proteins are central mediators of Wnt signaling pathways.
  • Their functions are complex due to multiple paralogs, maternal transcripts, and distinct Wnt pathway activation.
  • Understanding DVL's role in vertebrate early development and pathway specificity has been a long-standing challenge.

Purpose of the Study:

  • To review advances in understanding Dishevelled-mediated Wnt signaling during vertebrate gastrulation and neurulation.
  • To explore the structure-function relationships of Dishevelled domains.
  • To discuss the implications of Dishevelled isoforms in early development.

Main Methods:

  • Review of extensive studies in various animal models.
  • Analysis of structure-function relationships of conserved Dishevelled domains.
  • Examination of intra- and inter-molecular interactions and Dishevelled dosage effects.

Main Results:

  • Dishevelled proteins' conserved domains are key to Wnt signal transduction and cellular functions.
  • Dishevelled isoforms exhibit distinct and redundant roles in development and disease.
  • Spatiotemporal expression, biochemical properties, and post-translational modifications influence Dishevelled functions.

Conclusions:

  • Dishevelled proteins are critical for Wnt signaling specificity and early vertebrate development.
  • Interactions and dosage of Dishevelled proteins modulate Wnt pathway outcomes.
  • Further research on Dishevelled-mediated signaling offers insights into developmental processes and diseases.