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Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

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Coaxial nozzle-assisted electrohydrodynamic printing for microscale 3D cell-laden constructs.

Hongtao Liang¹, Jiankang He¹, Jinke Chang¹

¹State key laboratory for manufacturing systems engineering, Xi'an Jiaotong University, Xi'an, China.

International Journal of Bioprinting

|October 26, 2020

Summary

A new coaxial nozzle-assisted electrohydrodynamic cell printing strategy enables high-resolution, high-viability 3D living tissue fabrication. This advanced cell printing method achieves uniform hydrogel filaments and excellent cell survival rates.

Keywords:

biofabrication bioprinting cell printing electrohydrodynamic printing tissue engineering

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Last Updated: Dec 3, 2025

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

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Published on: September 27, 2019

Manufacturing of Three-dimensionally Microstructured Nanocomposites through Microfluidic Infiltration

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Published on: March 12, 2014

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer

Published on: September 22, 2015

Area of Science:

Biomedical Engineering
Tissue Engineering
Bioprinting

Background:

Cell printing is vital for fabricating living tissue constructs with controlled cell arrangements.
Current challenges include achieving high resolution and cell viability in 3D cell-laden structures.

Purpose of the Study:

To develop a coaxial nozzle-assisted electrohydrodynamic cell printing strategy for 3D cell-laden constructs.
To optimize printing parameters for improved resolution and cell viability.

Main Methods:

A coaxial nozzle-assisted electrohydrodynamic printing technique was employed.
Critical parameters like feeding rate and stage speed were evaluated to minimize hydrogel filament size.
The influence of CaCl2 feeding rate on alginate hydrogel printing was investigated.

Main Results:

The strategy successfully printed 3D hydrogel structures with uniform filament dimensions (approx. 80 μm).
Consistent cell distribution was achieved within the printed constructs.
Encapsulated cell viability remained above 90%.

Conclusions:

The coaxial nozzle-assisted electrohydrodynamic printing strategy offers a promising approach for fabricating 3D cell-laden constructs.
This method addresses key challenges in resolution and cell viability for advanced biomedical applications.