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Beyond bulk single-chain sequencing: Getting at the whole receptor
Nicholas C Curtis1, Jiwon Lee1
1Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755, United States.
Current Opinion in Systems Biology
|October 26, 2020
Summary
New paired B-cell receptor sequencing methods simplify antibody discovery. These high-throughput techniques enable better understanding of antibody responses against pathogens.
Area of Science:
- Immunology
- Molecular Biology
- Biotechnology
Background:
- Paired B-cell receptor sequencing is crucial for understanding antibody function.
- Existing methods can be complex and low-throughput.
Purpose of the Study:
- To develop simpler, high-throughput pipelines for native antibody heavy and light chain pair generation.
- To elucidate novel antibodies and antibody responses against pathogens.
Main Methods:
- Single-cell isolation using single wells or emulsified droplets.
- High-throughput sequencing of B-cell receptors.
- Integration of functional assays into workflows.
Main Results:
- Novel single-well and emulsion-based workflows improve throughput and reduce complexity.
- Functional characterization of pathogen-specific antibodies is enhanced.
- Immunological insights beyond bulk sequencing are revealed.
Conclusions:
- Advancements in paired B-cell receptor sequencing accelerate antibody discovery.
- These methods provide deeper insights into antibody-mediated immunity.
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