Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Lymphatic Vessels and Lymph Transport01:16

Lymphatic Vessels and Lymph Transport

21.4K
Lymphatic vessels, known as lymphatics, are crucial in transporting lymph from peripheral tissues to our venous system. This process begins with lymph entering through tiny capillaries that branch through tissues. These capillaries have unique features such as larger diameters, thinner walls, and a distinctive one-way valve system formed by overlapping endothelial cells.
This one-way system allows fluids, solutes, and even pathogens to enter but prevents their return to the intercellular...
21.4K
Detailed Structure and Function of Lymph Nodes01:23

Detailed Structure and Function of Lymph Nodes

3.7K
Lymph nodes are bean-shaped structures that cluster along the lymphatic vessels in the inguinal, axillary, and cervical regions. Each node is divided into compartments by a capsule that extends trabeculae inward.
From a histological perspective, lymph nodes can be split into two main areas: the superficial cortex and the deep medulla. The outer cortex is populated by dendritic cells, macrophages, and B lymphocytes, which are densely packed into follicles. When these B-lymphocytes are presented...
3.7K
Development of the Lymphatic System01:15

Development of the Lymphatic System

1.7K
The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
1.7K
Overview of the Vascular System01:20

Overview of the Vascular System

3.3K
The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
3.3K
Fluid Connective Tissues: Blood and Lymph01:20

Fluid Connective Tissues: Blood and Lymph

17.1K
Blood and lymph are fluid connective tissues. They contain cells, also known as formed elements, circulating in a liquid extracellular matrix, the plasma. The formed elements are derived from hematopoietic stem cells in the bone marrow. Blood and lymph connect all vital parts and carry nutrients, oxygen, and other essential molecules like antibodies.
Blood
The blood flows through blood vessels— arteries, capillaries, and veins. Blood plasma is primarily made of proteins, solutes, and...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reliability, repeatability, and age associations of the dogSIT (dog Smell Interaction Test) battery in untrained companion dogs (Canis lupus familiaris).

Scientific reports·2026
Same author

LYVE1 ectodomain shedding blunts lymphatic transmigration and clearance of macrophages during kidney injury.

JCI insight·2026
Same author

Caffeine-associated reduction in patent ductus arteriosus is mediated in part by adenosine receptor antagonism.

American journal of physiology. Heart and circulatory physiology·2025
Same author

Spatial Pathomics Toolkit for Quantitative Analysis of Podocyte Nuclei with Histology and Spatial Transcriptomics Data in Renal Pathology.

Proceedings of SPIE--the International Society for Optical Engineering·2025
Same author

Moving toward a better understanding of renal lymphatics: challenges and opportunities.

Pediatric nephrology (Berlin, Germany)·2025
Same author

Effects of Oral Macrocyclic Lactone Heartworm Preventatives on Retinal Function and Chromatic Pupillary Light Reflex in Healthy Companion Dogs.

Veterinary ophthalmology·2025

Related Experiment Video

Updated: Dec 3, 2025

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

16.6K

Renal lymphatic vessel dynamics.

Elaine L Shelton1,2, Hai-Chun Yang1,3, Jianyong Zhong1,3

  • 1Department of Pediatrics, Vanderbilt University Medical Center, Nashville, Tennessee.

American Journal of Physiology. Renal Physiology
|October 26, 2020
PubMed
Summary

Renal lymphatic vessels regulate fluid balance and respond to vasoactive factors. The diuretic furosemide impairs lymphatic function, potentially worsening fluid accumulation in certain conditions.

Keywords:
furosemideloop diureticspressure myographyrenal lymphatics

More Related Videos

Author Spotlight: Innovative Methods in Lymphedema and Hypertension Research
08:46

Author Spotlight: Innovative Methods in Lymphedema and Hypertension Research

Published on: March 22, 2024

1.5K
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

6.8K

Related Experiment Videos

Last Updated: Dec 3, 2025

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
09:52

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse

Published on: March 8, 2013

16.6K
Author Spotlight: Innovative Methods in Lymphedema and Hypertension Research
08:46

Author Spotlight: Innovative Methods in Lymphedema and Hypertension Research

Published on: March 22, 2024

1.5K
Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice
05:59

Blocking Lymph Flow by Suturing Afferent Lymphatic Vessels in Mice

Published on: May 14, 2020

6.8K

Area of Science:

  • Nephrology
  • Physiology
  • Vascular Biology

Background:

  • Renal lymphatics are crucial for fluid homeostasis, removing excess substances from the kidney interstitium.
  • Understanding renal lymphatic vessel function and the impact of drugs is limited.

Purpose of the Study:

  • To investigate the physiological properties of renal lymphatic vessels.
  • To examine the effects of diuretics on renal lymphatic function.

Main Methods:

  • Pressure myography was used to assess renal lymphatic vessel contractility and responses.
  • Vessel tone and spontaneous contractions were measured in response to transmural pressure and vasoactive factors.
  • The expression of Na+-K+-2Cl- cotransporter (Nkcc1) was investigated.

Main Results:

  • Renal lymphatic vessels exhibit pressure-dependent pumping and respond to prostaglandin E2 and nitric oxide.
  • Spontaneous contractility is dependent on calcium (Ca2+) and chloride (Cl-).
  • Furosemide caused dose-dependent dilation and reduced lymphatic pumping, while ethacrynic acid had no effect. Nkcc1 was found in extrarenal lymphatics.

Conclusions:

  • Renal lymphatic vessels possess unique physiological characteristics and contractility mechanisms.
  • Furosemide's inhibition of Nkcc1 impairs lymphatic vessel function, potentially exacerbating edema.
  • These findings highlight potential unintended consequences of furosemide treatment on fluid balance.