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Updated: Dec 3, 2025

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Published on: August 27, 2013

Deterministic droplet coding via acoustofluidics.

Peiran Zhang¹, Wei Wang, Hai Fu

¹Department of Mechanical Engineering and Material Science, Duke University, Durham, NC 27708, USA. tony.huang@duke.edu.

|October 26, 2020

Summary

This summary is machine-generated.

This study introduces an acoustofluidic platform for deterministic droplet coding, enabling on-demand, real-time labeling of microfluidic droplets. This breakthrough allows for high-capacity, error-tolerant barcoding for biomedical applications.

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Last Updated: Dec 3, 2025

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Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Published on: January 21, 2021

Area of Science:

Biomedical Engineering
Microfluidics
Acoustofluidics

Background:

Droplet microfluidics offers high throughput but lacks individual droplet identification.
Current methods struggle to label vast numbers of droplets for tracking.

Purpose of the Study:

To develop a method for deterministic, real-time coding of droplets based on volume.
To enable on-demand labeling and identification of individual droplets in microfluidic systems.

Main Methods:

Utilized an acoustofluidic platform with focused traveling surface acoustic waves.
Dynamically split aqueous flow using an oil jet to control droplet volume.
Dispensed sequences of droplets at 100 Hz with deterministic volumes.

Main Results:

Successfully encoded barcoding information using sequences of varying droplet lengths.
Demonstrated proof-of-concept with end-to-end packages containing address and sample barcodes.
Achieved high-capacity and high error-tolerance in droplet tagging.

Conclusions:

Acoustofluidics enables deterministic droplet coding for precise labeling.
This technique enhances droplet identification for high-throughput screening.
Potential applications include single-cell phenotyping and multiplexed assays.