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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

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Published on: April 6, 2016

On the Assembly of Microreactors with Parallel Enzymatic Pathways.

Adam Armada-Moreira^1,2,3, Bo Thingholm¹, Kristine Andreassen¹

¹Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000, Aarhus, Denmark.

Advanced Biosystems

|October 26, 2020

Summary

Researchers developed complex cell mimics by co-encapsulating multiple enzymes into microreactors. This advancement in artificial cell technology brings us closer to understanding biological processes and creating therapeutic cell mimics.

Keywords:

encapsulated catalysis enzymatic cascades enzymatic cycles microreactors

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Area of Science:

Biomimetic chemistry
Synthetic biology
Biotechnology

Background:

Cell mimicry seeks to replicate cellular functions using simpler artificial structures.
Increasing the complexity of cell mimics is crucial for advancing the field and understanding intracellular processes.

Purpose of the Study:

To develop more complex cell mimics by co-encapsulating multiple enzymatic pathways.
To demonstrate the feasibility of physically separating distinct enzymatic systems within microreactors.

Main Methods:

Assembly of compartmentalized microreactors using polymer layers and enzyme-loaded liposomal subunits.
Co-encapsulation of two distinct enzymatic pathways: a cycle involving glutamate dehydrogenase and glutathione reductase, and a cascade with β-galactosidase, glucose oxidase, and catalase.
Confirmation of enzymatic activity within the encapsulated systems, utilizing NADP+/NADPH as a common co-factor.

Main Results:

Successful co-encapsulation of up to five enzymes into compartmentalized microreactors.
Physical separation of two distinct enzymatic pathways within the microreactor system.
Demonstrated activity of both the encapsulated enzymatic cycle and cascade, confirming successful compartmentalization and function.

Conclusions:

This work represents a significant advancement in encapsulated catalysis.
The developed microreactor system enables the creation of more complex and functional cell mimics.
This approach holds promise for the future assembly of therapeutic cell mimics.