Sediminibacterium soli sp. nov., isolated from soil

Area of Science:

• Microbiology
• Bacterial Taxonomy
• Genomics

Background:

• The genus Sediminibacterium comprises bacteria isolated from various environments.
• Understanding bacterial diversity is crucial for ecological and biotechnological applications.

Purpose of the Study:

• To characterize a novel bacterial isolate from soil.
• To determine the taxonomic placement of the novel strain within the Sediminibacterium genus.

Main Methods:

• Isolation and cultivation of bacteria from soil.
• 16S rRNA gene sequencing and phylogenetic analysis.
• Genome sequencing, average nucleotide identity (ANI), and digital DNA-DNA hybridization (dDDH) calculations.
• Analysis of chemotaxonomic data (respiratory quinones, polar lipids, fatty acids).

Main Results:

• A novel Gram-negative, facultative anaerobic bacterium, designated WSJ-3, was isolated from soil.
• Phylogenetic analyses placed WSJ-3 within the Sediminibacterium genus, with highest 16S rRNA gene similarity to Sediminibacterium roseum (97.0%).
• Low ANI (72.2%) and dDDH (19.2%) values with S. roseum, along with distinct chemotaxonomic features, supported its classification as a new species.

Conclusions:

• The isolate WSJ-3 represents a novel species of the genus Sediminibacterium.
• The proposed name for the novel species is Sediminibacterium soli sp. nov.
• The type strain is WSJ-3 (KCTC 72839 = CCTCC AB 2019408).