A rapid, high-volume cervical screening project using self-sampling and isothermal PCR HPV testing.

Area of Science:

• Oncology
• Public Health
• Medical Technology

Background:

• Cervical cancer prevention requires scalable screening programs, particularly in high-burden regions like China.
• Human Papillomavirus (HPV) testing is a cornerstone of modern cervical cancer screening strategies.

Purpose of the Study:

• To evaluate a rapid, high-volume cervical cancer screening and treatment strategy in Inner Mongolia, China.
• To assess the feasibility of HPV self-sampling combined with same-day digital colposcopy and treatment.

Main Methods:

• A 5-day screening project involved 3345 women undergoing HPV self-sampling for rapid HPV testing using the AmpFire™ system.
• HPV-positive women received same-day digital colposcopy (DC) with the EVA™ system, followed by immediate treatment (thermal ablation or LLETZ) or referral based on colposcopic impression.

Main Results:

• Out of 3345 women screened, 18.7% (624) tested HPV-positive.
• 88.5% of HPV-positive women underwent same-day colposcopy, and 78 women received immediate treatment.
• Histology confirmed 20 cases of CIN2+, with 15 detected and managed during the screening event.

Conclusions:

• HPV self-sampling and rapid testing facilitate high-volume cervical cancer screening with minimal loss-to-follow-up.
• The portable AmpFire™ and EVA™ systems enable rapid HPV testing and visual triage, supporting efficient screen-and-treat strategies.
• Integrating HPV testing with visual inspection and same-day treatment is a viable approach for large-scale cervical cancer prevention.