Related Experiment Video
Updated: Dec 3, 2025
10:00
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
18.1K
Probing infectious disease by single-cell RNA sequencing: Progresses and perspectives
Geyang Luo1,2, Qian Gao2, Shuye Zhang1
1Shanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Computational and Structural Biotechnology Journal
|October 27, 2020
Abstract:
The increasing application of single-cell RNA sequencing (scRNA-seq) technology in life science and biomedical research has significantly increased our understanding of the cellular heterogeneities in immunology, oncology and developmental biology. This review will summarize the development of various scRNA-seq technologies; primarily discussing the application of scRNA-seq on infectious diseases, and exploring the current development, challenges, and potential applications of scRNA-seq technology in the future.
Keywords:
3C, Chromosome Conformation CaptureACE2, Angiotensin-Converting Enzyme 2ARDS, acute respiratory distress syndromeATAC-seq, Assay for Transposase-Accessible Chromatin using sequencingBCR, B cell receptorCEL-seq, Cell Expression by Linear amplification and SequencingCLU, clusterinCOVID-19, corona virus disease 2019CRISPR, Clustered Regularly Interspaced Short Palindromic RepeatsCytoSeq, gene expression cytometryDENV, dengue virusFACS, fluorescence-activated cell sortingGNLY, granulysinGO analysis, Gene Ontology analysisHIV, Human Immunodeficiency VirusIAV, Influenza A virusIGHV/HD/HJ/HC, Immune globulin heavy V/D/J/C/ regionIGLV/LJ/LC, Immune globulin light V/J/C/ regionILC, Innate Lymphoid CellInfectious diseasesLIGER, Linked Inference of Genomics Experimental RelationshipsMAGIC, Markov Affinity-based Graph Imputation of CellsMARS-seq, Massively parallel single-cell RNA sequencingMATCHER, Manifold Alignment To CHaracterize Experimental RelationshipsMCMV, mouse cytomegalovirusMERFISH, Multiplexed, Error Robust Fluorescent In Situ HybridizationMLV, Moloney Murine Leukemia VirusMOFA, Multi-Omics Factor AnalysisMOI, multiplicity of infectionPBMCs, peripheral blood mononuclear cellsPLAC8, placenta-associated 8SARS-CoV-2, severe acute respiratory syndrome coronavirus 2SAVER, Single-cell Analysis Via Expression RecoverySPLit-seq, split pool ligation-based tranome sequencingSTARTRAC, Single T-cell Analysis by RNA sequencing and TCR TRACkingSTRT-seq, Single-cell Tagged Reverse Transcription sequencingSingle-cell RNA sequencingTCR, T cell receptorTSLP, thymic stromal lymphopoietinUMAP, Uniform Manifold Approximation and ProjectionUMI, Unique Molecular IdentifiermcSCRB-seq, molecular crowding single-cell RNA barcoding and sequencingpDCs, plasmacytoid dendritic cellsscRNA-seq, single cell RNA sequencing technologysci-RNA-seq, single-cell combinatorial indexing RNA sequencingseqFISH, sequential Fluorescent In Situ Hybridizationsmart-seq, switching mechanism at 5′ end of the RNA transcript sequencingt-SNE, t-Distributed stochastic neighbor embedding
Related Concept Videos
03:21RNA-seq
11.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.3K
02:24Ribosome Profiling
3.9K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.9K