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Downregulating long non-coding RNA PVT1 expression inhibited the viability, migration and phenotypic switch of

Shouming Li1, Xin Zhao2, Shaopeng Cheng1

  • 1Department of Cardiovascular Surgery, Qilu Hospital of Shandong University, No.107, West Wenhua Road, Jinan, 250012, Shandong, China.

Human Cell
|October 27, 2020
PubMed

Insights

Long non-coding RNA PVT1 (Plasmacytoma Variant Translocation 1) down-regulation inhibits proliferation and migration in human aortic smooth muscle cells. This suggests PVT1 may be a therapeutic target for aortic dissection by modulating miR-27b-3p.

Area of Science:

  • Molecular Biology
  • Cardiovascular Biology
  • Genetics

Background:

  • Long non-coding RNA PVT1 (Plasmacytoma Variant Translocation 1) is implicated in various diseases.
  • The specific role of PVT1 in aortic dissection (AD) requires further investigation.
  • Understanding PVT1's function in human aortic smooth muscle cells (HASMCs) is crucial for AD research.

Purpose of the Study:

  • To investigate the role of LncRNA PVT1 in the pathogenesis of aortic dissection (AD).
  • To explore the interaction between PVT1 and microRNA-27b-3p (miR-27b-3p) in HASMCs.
  • To determine the therapeutic potential of targeting PVT1 in AD.

Main Methods:

  • Cell viability and migration assays (MTT, wound-healing) in HASMCs.
  • Quantitative real-time PCR (qRT-PCR) and Western blot for gene and protein expression analysis.
  • Establishment of an animal model for AD with pathological examination (H&E staining).

Main Results:

  • PVT1 expression was upregulated, while miR-27b-3p was downregulated in AD.
  • PVT1 competitively binds with miR-27b-3p in HASMCs.
  • Downregulating PVT1 reversed PDGF-BB-induced changes in HASMCs and suppressed AD-induced pathology in vivo.

Conclusions:

  • Downregulating PVT1 suppresses proliferation, migration, and phenotypic switching of HASMCs.
  • PVT1 targets miR-27b-3p to influence HASMC behavior in the context of AD.
  • PVT1 inhibition represents a potential therapeutic strategy for aortic dissection.
Keywords:
Aortic dissectionHuman aortic smooth muscle cells (HASMCs)Plasmacytoma Variant Translocation 1Platelet-derived growth factor-BBmicroRNA-27b-3p

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