The 'rejuvenating factor' TIMP-2 is detectable in human blood components for transfusion
Julia Hoefer1, Christian Dal-Pont2, Stefan Jochberger3
1Department of Urology, Medical University of Innsbruck, Innsbruck, Austria.
Vox Sanguinis
|October 27, 2020
Summary
Tissue inhibitor of metalloproteinases 2 (TIMP-2) is stable in blood components like plasma and platelets, but at lower levels in red blood cells. This suggests TIMP-2 may transfer to transfusion recipients.
Area of Science:
- Biochemistry
- Transfusion Medicine
- Protein Stability
Background:
- Tissue inhibitor of metalloproteinases 2 (TIMP-2) plays roles in tissue remodeling and metastasis suppression.
- TIMP-2 administration improved cognitive function in aged mice, suggesting a potential role in human cognition.
- Investigating TIMP-2 stability in blood products is crucial for understanding its potential impact on transfusion recipients.
Purpose of the Study:
- To determine TIMP-2 concentrations in fresh-frozen plasma (FFP), erythrocyte concentrate (EC), and pathogen-inactivated platelet concentrate (PI-PC).
- To assess the influence of donor age and gender on TIMP-2 levels in blood components.
- To evaluate the stability of TIMP-2 in different blood products used for transfusion.
Main Methods:
- TIMP-2 concentrations were measured using a Q-Plex immunoassay with chemiluminescence detection.
- Absolute quantification of TIMP-2 was performed using Q-view software.
- Samples included FFP (n=30), EC (n=12), and PI-PC (n=12), with fresh umbilical cord plasma as a positive control.
Main Results:
- TIMP-2 was detected in all tested blood components (FFP, EC, PI-PC).
- Median TIMP-2 concentration was significantly lower in EC (17.2 ng/ml) compared to FFP (63.4 ng/ml) and PI-PC (69.9 ng/ml).
- TIMP-2 levels were comparable between male and female donors and were independent of age.
Conclusions:
- TIMP-2 is detectable and stable in FFP and PI-PC, and present at lower concentrations in EC.
- The findings suggest TIMP-2 can be transmitted to transfusion recipients.
- Further research may explore the clinical implications of TIMP-2 transfusion on recipient outcomes.
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