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Gut mucosa in the rats exposed temporary mechanical obstruction fed with probiotic
Akay Edizsoy1, Eyüp Yılmaz2, Mehmet Hakan Çevikel2
1Departmant of General Surgery, Surgical Oncology, Mersin University Faculty of Medicine, Mersin-Turkey.
Background:
Created a model in the rats, to prevent mucosal damage and related effects in the patients, who were operated due to mechanical obstruction. Some groups fed fodder with probiotics, some groups fed with standard fodder. It is objected that the damage of gut mucosa and related effects on how to expose the differences of the groups.
Methods:
In this study, 48 female Wistar-albino type rats are separated into five groups randomly. In the first operation, rats' terminal ileum was tied up with silk except for the control group. Two groups 24, the other two groups 48 hours later operated again and terminal ileum obstructions were removed. During that time, each one of those 24 and 48 hours of obstructed groups were fed with probiotic. Twenty-four hours later, the control group and other groups were operated for the third time for sampling. Terminal ileum, liver, spleen, MLN (Mesenteric lymph node) and blood samples were taken.
Results:
The research group, which was obstructed and fed with probiotics during 48 hours, was significantly observed in increased mucosa cell loss and mucosal edema. Bacterial translocation was found more common in groups without probiotics. Tissue GR (Glutathione reductase) and erythrocyte CAT (Catalase) were lower in the group of 24 hours obstructed and given probiotics.
Conclusion:
The findings suggest that the high rate of mucosal edemas in the groups that are fed with probiotics can be seen as damage, but we think that probiotics are consonant with the strength of the mucosal barrier. Thus, in the groups fed with probiotics, it is possible that bacterial translocation is seen less, and some antioxidative enzymes are found less. Further studies are needed to investigate the benefits of probiotics in patients operated for obstruction.
Insights
Probiotics may help strengthen the gut mucosal barrier after intestinal obstruction surgery in rats. While some groups showed increased edema, probiotic use reduced bacterial translocation, suggesting a protective effect.
Area of Science:
- Gastroenterology
- Surgical Research
- Microbiology
Background:
- Intestinal obstruction surgery can lead to mucosal damage.
- The role of probiotics in mitigating these effects is not fully understood.
- A rat model was developed to investigate probiotic impact on gut mucosa.
Purpose of the Study:
- To evaluate the effect of probiotics on gut mucosal integrity and bacterial translocation in a rat model of intestinal obstruction.
- To compare the outcomes between rats fed probiotics and those fed standard fodder.
Main Methods:
- Forty-eight Wistar-albino rats were randomly assigned to five groups.
- Surgical induction of terminal ileum obstruction followed by removal.
- Probiotic or standard fodder administration during obstruction periods (24 or 48 hours).
- Sample collection included terminal ileum, liver, spleen, mesenteric lymph node, and blood.
Main Results:
- The 48-hour obstructed group receiving probiotics showed increased mucosal cell loss and edema.
- Bacterial translocation was more prevalent in groups not receiving probiotics.
- Lower levels of glutathione reductase (GR) and catalase (CAT) were observed in the 24-hour obstructed probiotic group.
Conclusions:
- Probiotic administration may enhance mucosal barrier strength, reducing bacterial translocation despite potential edema.
- Observed decreases in certain antioxidative enzymes warrant further investigation.
- Probiotics show potential benefits for patients undergoing surgery for intestinal obstruction.
