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Updated: Aug 31, 2026

07:38
Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
Arsenate-resistant alkaline phosphatase-constitutive mutants of Escherichia coli
Abstract:
When arsenate-resistant mutants are selected approximately 50 per cent of them are also consitutive for the synthesis of alkaline phosphatase and the Pi-binding protein. Some of these mutants are linked to ilv (phoS- or phoT-), other are linked to proC (phoR-). One of the mutant strains linked to ilv lost the Pi-binding protein (the phoS gene product). Resistance to arsenate, constitutivty for alkaline phosphatase synthesis and loss of the Pi-binding protein occurred pleiotropically by the same phoS- mutation.
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