Role of aposomes and epididymosomes in sperm quality control: A light and transmission electron microscopic study in...

The epididymis responds to adverse conditions of misshapen spermatozoa resulting from pathological changes or toxic insults by secretion of a dense matrix that segregates the latter for complete disintegration and dissolution. The objective of this study was to find the source of this matrix and the role-player of disintegration and dissolution of misshapen spermatozoa. We chose Wistar strain male rat model to tackle this issue, and the rats were administered with aflatoxin B1 for 55 days so as to increase the incidence of misshapen spermatozoa. At the end of the treatment, different segments of epididymis were processed for microscopic observations. We found that parallel with abundant misshapen spermatozoa in the epididymis the principal cells of the initial segment secrete enormous membrane-bound apical blebs called aposomes, which contain epididymosomes. The aposomes were found to coalesce so as for the content to merge and form a dense matrix that entangles the misshapen spermatozoa and segregates them from viable spermatozoa. The epididymosomes associate with the misshapen spermatozoa, and the latter is processed to disintegration and total dissolution. Therefore, we assign the role of segregation of misshapen spermatozoa from viable ones to the dense matrix of aposomes and their disintegration and dissolution to the epididymosomes.