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Remineralization of enamel subsurface lesions using toothpaste containing tricalcium phosphate and fluoride: an in
Hidenori Hamba1,2, Keiki Nakamura3, Toru Nikaido4
1Department of Operative Dentistry, Cariology and Pulp Biology, Tokyo Dental College, 2-9-18, Kanda-Misakicho, Chiyoda-ku, Tokyo, 101-0061, Japan. hambah@tdc.ac.jp.
Background:
This study aimed to compare the efficacies of experimental toothpastes containing functionalized tricalcium phosphate (fTCP) with and without fluoride for in vitro enamel remineralization under pH-cycling conditions.
Methods:
To create artificial white spot lesions, 36 bovine enamel specimens were immersed in a demineralization solution for 10 days. During pH-cycling for 12 days, the specimens were divided into four groups based on the experimental toothpaste type used: (a) fTCP-free, fluoride-free (fTCP - F -); (b) fTCP-containing, fluoride-free (fTCP + F -); (c) fTCP-free, fluoride-containing (fTCP - F +); and (d) fTCP-containing, fluoride-containing (fTCP + F +). Micro-focus X-ray computed tomography (μCT) scans of all specimens were obtained before demineralization, after demineralization, and after pH-cycling. The mineral density and mineral loss (ΔZ) in the enamel subsurface lesions were measured and the percentage of remineralization (%R) was calculated from ΔZ after demineralization and pH-cycling. One-way ANOVA with Tukey's test was used for statistical analysis of the %R values. The treated enamel surface was investigated via scanning electron microscopy (SEM).
Results:
The fTCP - F - group presented with the lowest amount of mineral gain after pH-cycling. In contrast, the fTCP + F + group showed the highest degree of remineralization within all lesion parts. The %R was highest in the fTCP + F + group (38.2 ± 7.8, all P < 0.01). SEM revealed the presence of small crystals on the enamel rods in the fTCP + F - and fTCP + F + groups.
Conclusions:
The experimental toothpaste containing fTCP and fluoride increased remineralization of the artificial enamel subsurface lesions during pH-cycling. Furthermore, fTCP and fluoride appear to act independently on the remineralization of enamel subsurface lesions, although they coexisted in one toothpaste type.
Trial Registration:
This is not a human subject research.
