Conjugation of Carboxylated Graphene Quantum Dots with Cecropin P1 for Bacterial Biosensing Applications

Biosensors that can accurately and rapidly detect bacterial concentrations in solution are important for potential applications such as assessing drinking water safety. Meanwhile, quantum dots have proven to be strong candidates for biosensing applications in recent years because of their strong light emission properties and their ability to be modified with a variety of functional groups for the detection of different analytes. Here, we investigate the use of conjugated carboxylated graphene quantum dots (CGQDs) for the detection of Escherichia coli using a biosensing assay that focuses on measuring changes in fluorescence intensity. We have further developed this assay into a novel, compact, field-deployable biosensor focused on rapidly measuring changes in absorbance to determine E. coli concentrations. Our CGQDs were conjugated with cecropin P1, a naturally produced antibacterial peptide that facilitates the attachment of CGQDs to E. coli cells; to our knowledge, this is the first instance of cecropin P1 being used as a biorecognition element for quantum dot biosensors. As such, we confirm the structural modification of these conjugated CGQDs in addition to analyzing their optical characteristics. Our findings have the potential to be used in situations where rapid, reliable detection of bacteria in liquids, such as drinking water, is required, especially given the low range of E. coli concentrations (10³ to 10⁶ CFU/mL) within which our two biosensing assays have collectively been shown to function.