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Related Experiment Video

Updated: Dec 3, 2025

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Chromatin-Bound Proteome Profiling by Genome Capture.

Sergi Aranda1, Eva Borràs1,2, Eduard Sabidó1,2

  • 1Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain.

STAR Protocols
|October 28, 2020
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Summary
This summary is machine-generated.

This study introduces a new method, isolation of proteins on total DNA (iPOTD), for unbiased identification of proteins bound to DNA. This technique aids in discovering novel cellular pathways and disease-related interactions.

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Area of Science:

  • Molecular Biology
  • Proteomics
  • Genomics

Background:

  • Identifying proteins interacting with chromatin is crucial for understanding cellular pathways and disease mechanisms.
  • Existing methods like DNA mediated chromatin pull-down (Dm-ChP) focus on nascent DNA, limiting comprehensive analysis.

Purpose of the Study:

  • To present a detailed, step-by-step protocol for the isolation of proteins on total DNA (iPOTD).
  • To enable robust and unbiased surveying of the cellular chromatin-bound proteome.
  • To facilitate *de novo* identification of chromatin interactors.

Main Methods:

  • The study details a novel experimental protocol, isolation of proteins on total DNA (iPOTD).
  • This method is inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology.
  • The protocol is designed for bulk chromatome profiling.

Main Results:

  • The isolation of proteins on total DNA (iPOTD) protocol allows for unbiased identification of chromatin-bound proteins.
  • This method provides a comprehensive survey of the cellular chromatin-bound proteome.
  • The protocol is described as simple and robust.

Conclusions:

  • The isolation of proteins on total DNA (iPOTD) protocol offers a powerful tool for *de novo* identification of chromatin interactors.
  • This technique can reveal unexpected pathways relevant to physiology and human disease.
  • The protocol facilitates broad chromatome profiling.