Cell-Based Glycan Arrays-A Practical Guide to Dissect the Human Glycome
Christian Büll1, Hiren J Joshi1, Henrik Clausen1
1Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark.
Abstract:
Exploring the biological functions of the human glycome is highly challenging given its tremendous structural diversity. We have developed stable libraries of isogenic HEK293 cells with loss or gain of glycosylation features that together form the cell-based glycan array, a self-renewable resource for the display of the human glycome in the natural context. This protocol describes the use of the cell-based glycan array for dissection of molecular interactions and biological functions of glycans using a wide range of biological assays. For complete details on the use and execution of this protocol, please refer to (Narimatsu et al., 2019).
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