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Updated: Dec 3, 2025
Isolation of Murine Lymph Node Stromal Cells
Published on: August 19, 2014
Single-Cell Transcriptomics of Human Lymph Node Stroma
Akira Takeda1, Sirpa Jalkanen1
1MediCity Research Laboratory and Institute of Biomedicine, University of Turku, Turku, Finland.
Abstract:
Accumulating evidence indicates that the immune system is regulated not only by immune cells but also by stromal cells in the tissue microenvironment. Characterization of non-hematopoietic cells has not been performed in depth, since markers of the subsets are limited. Recent advances of single-cell technology allow researchers to characterize comprehensively the heterogeneity of stromal cells in an unbiased manner. In this article, we provide step-by-step protocols for cell preparation for single-cell RNA sequencing to characterize the heterogeneity of stroma in human lymph nodes. For complete details on the use and execution of this protocol, please refer to Takeda et al. (2019).
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