Related Experiment Video
Updated: Dec 3, 2025
Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro
Published on: June 2, 2018
Generation of Pancreatic Organoid-Derived Isografts
Sabrina D'Agosto1, Francesca Lupo1, Vincenzo Corbo1
1Department of Diagnostics and Public Health, University of Verona, Verona 37134, Italy.
Abstract:
This protocol is a procedure for generating orthotopic isografts using mouse pancreatic cancer organoids. These isografts can be used to track the evolution of pancreatic ductal adenocarcinoma (PDA) from a preinvasive lesion to a metastatic disease and therefore represent a suitable model for identification of determinants of PDA progression. For complete details on the use and execution of this protocol, please refer to Boj et al. (2015) and Filippini et al. (2019).
