Protocol for the Maintenance of Quiescent Murine Hematopoietic Stem Cells

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Hiroshi Kobayashi1, Keiyo Takubo1

  • 1Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.

STAR Protocols
|October 28, 2020
PubMed

Mammalian hematopoietic stem cells (HSCs) maintain life-long hematopoiesis in the bone marrow. HSCs remain quiescent in vivo, unlike more differentiated progenitors, and enter the cell cycle rapidly after bone marrow injury or in vitro culture. We have recently demonstrated the ability to maintain HSC quiescence in vitro by mimicking the bone marrow microenvironment. Here, we provide a detailed protocol for keeping functional HSCs in the quiescent state in vitro. For complete details on the use and execution of this protocol, please refer to Kobayashi et al. (2019).

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