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Protocol for Isolation of Golgi Vesicles from Human and Animal Hearts by Flotation through a Discontinuous Sucrose
Estefanía Tarazón1, Manuel Portolés1, Esther Roselló-Lletí1
1Myocardial Dysfunction and Cardiac Transplantation Group and CIBERCV, Health Research Institute of La Fe University Hospital (IIS La Fe), Valencia 46026, Spain.
Abstract:
Detailed study of cellular organelles requires their isolation. Several protocols have been described for the isolation of the Golgi apparatus from liver tissue, but these are not suitable and not reproducible in harder tissues. Here, we describe a protocol to isolate Golgi vesicles from cardiac tissue using a discontinuous sucrose gradient. For complete details on the use and execution of this protocol, please refer to Tarazon et al. (2017).
