Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Induced erythroleukemia differentiation: cellular and molecular aspects.

R A Rifkind1, M Sheffery, P A Marks

  • 1DeWitt Wallace Research Laboratories, Memorial Sloan-Kettering Cancer Center, New York.

Blood Cells
|January 1, 1987
PubMed
Summary

Mouse erythroid lineage cells (MELC) differentiate into red blood cells when treated with HMBA. Early changes include decreased protein kinase C activity and proto-oncogene suppression before commitment to differentiation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical spectrum of hemolytic anemia associated with glucose-6-phosphate dehydrogenase deficiency.

Annals of internal medicine·2013
Same author

Excess alpha chain synthesis relative to beta chain synthesis in thalassaemia major and minor.

Nature·2010
Same author

THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : I. THE CELLULAR SURFACE.

The Journal of experimental medicine·2009
Same author

THE APPLICATION OF FERRITIN-CONJUGATED ANTIBODY TO ELECTRON MICROSCOPIC STUDIES OF INFLUENZA VIRUS IN INFECTED CELLS : II. THE INTERIOR OF THE CELL.

The Journal of experimental medicine·2009
Same author

THE INTRACELLULAR DISTRIBUTION OF GAMMA GLOBULIN IN A MOUSE PLASMA CELL TUMOR (X5563) AS REVEALED BY FLUORESCENCE AND ELECTRON MICROSCOPY.

The Journal of experimental medicine·2009
Same author

Histone deacetylase inhibitors: Potential in cancer therapy.

Journal of cellular biochemistry·2009

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Hematopoiesis

Background:

  • Terminal erythroid differentiation is a complex process.
  • The molecular mechanisms initiating and regulating this process in mouse erythroid lineage cells (MELC) are not fully understood.

Purpose of the Study:

  • To investigate the early molecular events and gene expression changes during HMBA-induced erythroid differentiation in MELC.

Main Methods:

  • Analysis of cell function and gene expression during the latent and commitment phases of differentiation.
  • Monitoring of diacylglycerol concentration, protein kinase C activity, and proto-oncogene transcription (c-myb, c-myc, c-fos).
  • Quantification of globin gene transcription and rRNA synthesis.

Main Results:

Related Experiment Videos

  • A latent period precedes differentiation commitment, marked by decreased diacylglycerol and protein kinase C activity.
  • Suppression of c-myb and c-myc transcription, decreased p53 protein, and increased c-fos mRNA occur during the latent phase.
  • Commitment at 12 hours correlates with sustained c-myb suppression and a 10-30 fold increase in alpha 1 and beta maj globin gene transcription.
  • rRNA synthesis is suppressed, with other gene expression changes yet to be fully characterized.

Conclusions:

  • Early molecular events, including protein kinase C modulation and proto-oncogene regulation, play critical roles in initiating erythroid differentiation.
  • Further research is needed to elucidate the precise regulatory roles of these early changes in sustaining the differentiation process.