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Related Experiment Video

Updated: Dec 3, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Practical guide for circular RNA analysis: Steps, tips, and resources.

Dimitrios Tsitsipatis¹, Myriam Gorospe¹

¹Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, Baltimore, Maryland, USA.

Wiley Interdisciplinary Reviews. RNA

|October 28, 2020

Summary

This summary is machine-generated.

Circular RNAs (circRNAs) are a novel class of transcripts with unique functions, often interacting with microRNAs and proteins to influence cellular processes and disease. This study offers guidelines for circRNA research, covering detection, partner identification, and analysis.

Keywords:

RNA-RNA interaction circular RNA ribonucleoprotein complex

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Last Updated: Dec 3, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Area of Science:

Molecular Biology
Genomics
RNA Biology

Background:

Recent advances in RNA sequencing enable detailed study of circular RNAs (circRNAs).
circRNAs exhibit unique functions, often through interactions with microRNAs and RNA-binding proteins.
These interactions link circRNAs to critical cellular processes and various disease pathologies.

Purpose of the Study:

To provide comprehensive guidelines for developing robust circRNA research studies.
To address key considerations in circRNA detection, characterization, and functional analysis.
To facilitate standardized and effective investigation of circRNA roles in biology and disease.

Main Methods:

Guidelines cover circRNA detection and selection strategies.
Methods for identifying circRNA binding partners and interaction sites are discussed.
Approaches for modulating circRNA levels and assessing stoichiometry are detailed.

Main Results:

The study outlines critical parameters for circRNA study design.
It addresses unique challenges in analyzing circRNA copy numbers and stoichiometry.
Recommendations are provided for robust circRNA investigation.

Conclusions:

Standardized guidelines are essential for advancing circRNA research.
Effective circRNA analysis requires careful consideration of detection, interaction, and quantification methods.
This work aims to improve the reliability and impact of studies on circular RNAs and their roles in health and disease.