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Published on: March 11, 2014
Dental Calculus as a Potential Biosource for Human Papillomavirus Detection in Oral Squamous Cell Carcinoma
Natallia Pranata1,2, Ani Melani Maskoen3, Edhyana Sahiratmadja4
1Graduate School of Biomedical Sciences, Master Program, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.
Objective:
The infection of human papillomaviruses (HPVs) plays a role in the development of oral squamous cell carcinoma (OSCC). A poor oral hygiene and dental calculus may cause the infection to persist. Therefore, this study aimed to assess whether this dental calculus could serve as a potential biosource in early detection of HPVs in patients with OSCC.
Methods:
DNA was isolated from the dental calculus of people diagnosed with OSCC, and MY09/11 primer set was used to detect the presence of HPV. The positive samples were further sequenced and aligned using megablast NCBI BLAST tool to identify the HPV genotype.
Results:
Electrophoresis examination showed that 4 of 14 samples collected (29%) had a clear single band, of which three had 97% to 99% similarity to a high-risk genotype HPV-58. Meanwhile, the other sample had 99% similarity to an unclassified papillomaviridae.
Conclusion:
Dental calculus is a promising source of HPV in oral cavity and could be used as a biomarker for early detection.
