Endolithic Microbial Carbon Cycling in East Antarctica

Antarctica is an ideal analogue for studying the limits of life. Despite severe temperature fluctuations and desiccating conditions, life is commonly found colonizing the structural cavities within Antarctic rocks (i.e., endoliths). Previous studies have speculated that the slow cycling of endoliths in the McMurdo Dry Valleys may be the limit of life on Earth. However, very little is known about the in situ activities of these communities-especially in regions outside the McMurdo Dry Valleys where endoliths are thought to be cycling carbon very slowly (e.g., hundreds of years). Here, we show that East Antarctic endoliths found on nunataks are cycling carbon quickly and are therefore quite active. Through radiocarbon (¹⁴C) analyses of the viable cell membrane (as phospholipid-derived fatty acids [PLFA]), we found that the Δ¹⁴C composition of these microbial communities was on average predominantly modern, with a few samples signaling older carbon in the system. These findings indicate that endoliths inhabiting inland Antarctic nunataks are cycling carbon on decadal timescales, which support the notion that endoliths in Antarctica are cycling carbon quickly. This work provides new insights into the potential variability of Antarctic endolith activities and demonstrates that, despite the climatic extremes that exist farther inland on the most inhospitable continent on Earth, indigenous life can thrive.