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Impaired thiol/disulphide homoeostasis in children with steroid-sensitive nephrotic syndrome

Kenan Yılmaz1, Gökhan Çakırca2, Özcan Erel3

  • 1Department of Pediatric Nephrology, Sanliurfa Training and Research Hospital, Sanliurfa, Turkey.

International Journal of Clinical Practice
|October 28, 2020
PubMed

Insights

Children with steroid-sensitive nephrotic syndrome (SSNS) show altered thiol/disulphide homoeostasis (TDH) during remission, indicating increased oxidative stress. This imbalance may contribute to the condition

Area of Science:

  • Biochemistry
  • Pediatric Nephrology
  • Oxidative Stress Research

Background:

  • Abnormal thiol/disulphide homoeostasis (TDH) is implicated in various disease pathologies.
  • Investigating TDH in pediatric nephrotic syndromes is crucial for understanding disease mechanisms.

Purpose of the Study:

  • To evaluate the thiol/disulphide homoeostasis (TDH) in children diagnosed with steroid-sensitive nephrotic syndrome (SSNS).
  • To determine if altered TDH is present in SSNS patients during remission.

Main Methods:

  • A cohort of 131 children (60 with SSNS, 71 healthy controls) was studied.
  • Plasma levels of total thiol (TT), native thiol (SH), and disulphide (SS) were measured using a novel assay.
  • Comparison of TDH parameters between SSNS patients in remission and healthy controls.

Main Results:

  • Children with SSNS exhibited decreased levels of albumin, total thiol (TT), and native thiol (SH), along with a lower SH/TT ratio.
  • Elevated disulphide/thiol ratios (SS/SH and SS/TT) were observed in the SSNS group.
  • Albumin levels showed a positive correlation with TT, SH, and SS levels in SSNS patients.

Conclusions:

  • Thiol/disulphide homoeostasis (TDH) is shifted towards oxidants in children with SSNS during remission.
  • This shift suggests that SSNS patients experience heightened oxidative stress even in remission.
Abstract

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