Interactive effects of mercuric oxide nanoparticles and future climate CO₂ on maize plant

So far, the phytotoxic hazards of nano-sized mercuric oxide (HgO-NPs) are not investigated. Herein, the phytotoxicity of fully characterized HgO-NPs (100 mg/kg soil), prepared by coprecipitation method, on maize grown under ambient (aCO₂, 410 ppm) and elevated CO₂ (eCO₂, 620 ppm) was investigated. Regardless of CO₂ concentration, HgO-NPs treatment increased Hg levels in maize organs. HgO-NPs induced severe oxidative stress in aCO₂ grown plants as indicated by reduced growth and photosynthesis and accumulation of reactive oxygen species (ROS), through photorespiration and nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activities, and lipid and protein oxidation products. Although HgO-NPs increased molecular (polyphenols, flavonoids, tocopherols) and enzymatic (superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione peroxidase) antioxidants in shoots of aCO₂ plants, but this failed to fight the eruption of increased ROS. On contrary, eCO₂ treatment mitigated the HgO-NPs impact by promoting photosynthesis and reducing the Hg-induced ROS production. Moreover, eCO₂ promoted ROS detoxification via molecular antioxidants overproduction, enhanced superoxide dismutase, catalase and peroxidases activities, and modulation of reduced ascorbate/oxidized ascorbate and reduced glutathione/oxidized glutathione homeostasis. The combined HgO-NPs + eCO₂ treatment also enhanced the glutathione-S-transferase activity. This study suggests that HgO-NPs cause severe phytotoxic hazards and this effect will be less detrimental under future CO₂ climate.