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Live Cell Fluorescence Microscopy to Observe Essential Processes During Microbial Cell Growth
Published on: November 24, 2017
From Differential Stains to Next Generation Physiology: Chemical Probes to Visualize Bacterial Cell Structure
Jonathan Hira1, Md Jalal Uddin1, Marius M Haugland2
1Research Group for Host-Microbe Interactions, Department of Medical Biology and Centre for New Antibacterial Strategies (CANS), UiT-The Arctic University of Norway, 9019 Tromsø, Norway.
Abstract:
Chemical probes have been instrumental in microbiology since its birth as a discipline in the 19th century when chemical dyes were used to visualize structural features of bacterial cells for the first time. In this review article we will illustrate the evolving design of chemical probes in modern chemical biology and their diverse applications in bacterial imaging and phenotypic analysis. We will introduce and discuss a variety of different probe types including fluorogenic substrates and activity-based probes that visualize metabolic and specific enzyme activities, metabolic labeling strategies to visualize structural features of bacterial cells, antibiotic-based probes as well as fluorescent conjugates to probe biomolecular uptake pathways.
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