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Updated: Dec 3, 2025

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

Quantitative analysis of hidden particles diffusing behind a scattering layer using speckle correlation.

Anirban Sarkar, Irène Wang, Jörg Enderlein

|October 29, 2020

Summary

This summary is machine-generated.

Speckle-correlation imaging quantifies dynamic fluorescent beads on surfaces. This method determines particle diffusion and count without phase retrieval, even with thick scattering layers.

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Area of Science:

Optics and Photonics
Biophysics
Materials Science

Background:

Speckle-correlation imaging utilizes the "memory effect" to visualize objects through opaque layers.
Dynamic processes, like particle diffusion, present unique challenges for imaging through scattering media.

Purpose of the Study:

To apply correlation analysis to quantitative imaging of dynamic fluorescent beads.
To determine particle diffusion constants and counts using speckle patterns.
To assess the applicability of the method for varying scattering layer thicknesses.

Main Methods:

Utilized an epi-fluorescence microscope with speckled illumination and detection.
Calculated spatio-temporal cross-correlation of detection speckle patterns.
Analyzed correlation as a function of lag time and spatial shift.

Main Results:

Successfully quantified the diffusion constant of fluorescent beads.
Determined the number of fluorescent particles without phase retrieval.
Demonstrated method utility even when the "memory effect" range is limited.

Conclusions:

Correlation analysis of speckle patterns provides a robust method for quantitative imaging of dynamic systems.
The technique is effective for measuring particle diffusion and concentration.
The method's independence from the "memory effect" range broadens its applicability to diverse scattering scenarios.