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Poly-3-hydroxybutyrate/chitosan composite films and nonwoven mats.

A A Sukhanova1, A E Murzova2, A N Boyandin3

  • 1Reshetnev Siberian State University of Science and Technology, 31 Krasnoyarsky Rabochy Av., Krasnoyarsk 660037, Russia; Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.

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Summary

This study developed poly-3-hydroxybutyrate (P(3HB)) and chitosan composite films and nonwoven mats. The composites exhibited enhanced hydrophilicity, mechanical strength, and supported NIH 3T3 fibroblast cell attachment and growth.

Keywords:
ChitosanCompositesFilmsNonwoven matsPhysicochemical propertiesPoly-3-hydroxybutyrateTissue engineering

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Area of Science:

  • Polymer Science
  • Biomaterials Engineering
  • Materials Science

Background:

  • Poly-3-hydroxybutyrate (P(3HB)) is a biodegradable polyester with potential biomedical applications.
  • Chitosan is a natural polysaccharide known for its biocompatibility and bioactivity.
  • Developing composite materials from P(3HB) and chitosan can leverage their synergistic properties for advanced applications.

Purpose of the Study:

  • To prepare and characterize composite films and nonwoven mats of P(3HB) and chitosan.
  • To investigate the effect of chitosan addition on the physical, mechanical, and surface properties of P(3HB).
  • To evaluate the in vitro biodegradation and cell interaction capabilities of the P(3HB)-chitosan composites.

Main Methods:

  • Differential Scanning Calorimetry (DSC) and Fourier-Transform Infrared Spectroscopy (FTIR) for thermal and structural analysis.
  • Scanning Electron Microscopy (SEM) for surface morphology and fiber diameter analysis.
  • Water contact angle measurements for surface hydrophilicity assessment.
  • In vitro biodegradation tests and NIH 3T3 fibroblast cell culture for biocompatibility evaluation.

Main Results:

  • P(3HB) blending with chitosan decreased P(3HB) crystallinity to 47% (films) and 62% (nonwoven mats).
  • Chitosan addition altered surface morphology and reduced ultrafine fiber diameter to 460 nm in nonwoven mats.
  • Composites showed enhanced hydrophilic properties (water contact angle ~50-53°) and improved mechanical strength.
  • The P(3HB)-chitosan composites demonstrated good in vitro biodegradation and supported NIH 3T3 fibroblast cell attachment and growth.

Conclusions:

  • Composite films and nonwoven mats of P(3HB) and chitosan were successfully prepared.
  • The composites exhibit tunable properties, including enhanced hydrophilicity and mechanical reinforcement.
  • These P(3HB)-chitosan biomaterials show promise for applications requiring biocompatibility, biodegradability, and good cell interaction.