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Poly-3-hydroxybutyrate/chitosan composite films and nonwoven mats
A A Sukhanova1, A E Murzova2, A N Boyandin3
1Reshetnev Siberian State University of Science and Technology, 31 Krasnoyarsky Rabochy Av., Krasnoyarsk 660037, Russia; Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia.
International Journal of Biological Macromolecules
|October 29, 2020
Summary
This study developed poly-3-hydroxybutyrate (P(3HB)) and chitosan composite films and nonwoven mats. The composites exhibited enhanced hydrophilicity, mechanical strength, and supported NIH 3T3 fibroblast cell attachment and growth.
Area of Science:
- Polymer Science
- Biomaterials Engineering
- Materials Science
Background:
- Poly-3-hydroxybutyrate (P(3HB)) is a biodegradable polyester with potential biomedical applications.
- Chitosan is a natural polysaccharide known for its biocompatibility and bioactivity.
- Developing composite materials from P(3HB) and chitosan can leverage their synergistic properties for advanced applications.
Purpose of the Study:
- To prepare and characterize composite films and nonwoven mats of P(3HB) and chitosan.
- To investigate the effect of chitosan addition on the physical, mechanical, and surface properties of P(3HB).
- To evaluate the in vitro biodegradation and cell interaction capabilities of the P(3HB)-chitosan composites.
Main Methods:
- Differential Scanning Calorimetry (DSC) and Fourier-Transform Infrared Spectroscopy (FTIR) for thermal and structural analysis.
- Scanning Electron Microscopy (SEM) for surface morphology and fiber diameter analysis.
- Water contact angle measurements for surface hydrophilicity assessment.
- In vitro biodegradation tests and NIH 3T3 fibroblast cell culture for biocompatibility evaluation.
Main Results:
- P(3HB) blending with chitosan decreased P(3HB) crystallinity to 47% (films) and 62% (nonwoven mats).
- Chitosan addition altered surface morphology and reduced ultrafine fiber diameter to 460 nm in nonwoven mats.
- Composites showed enhanced hydrophilic properties (water contact angle ~50-53°) and improved mechanical strength.
- The P(3HB)-chitosan composites demonstrated good in vitro biodegradation and supported NIH 3T3 fibroblast cell attachment and growth.
Conclusions:
- Composite films and nonwoven mats of P(3HB) and chitosan were successfully prepared.
- The composites exhibit tunable properties, including enhanced hydrophilicity and mechanical reinforcement.
- These P(3HB)-chitosan biomaterials show promise for applications requiring biocompatibility, biodegradability, and good cell interaction.
