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The Novel Circular RNA Circ-PGAP3 Promotes the Proliferation and Invasion of Triple Negative Breast Cancer by

Dabao He1, Xiaoling Yang2, Wenbin Kuang1

  • 1Department of Laboratory Medicine, Shenzhen Longhua District Central Hospital, Shenzhen 518110, People's Republic of China.

Oncotargets and Therapy
|October 29, 2020
PubMed
Abstract

Insights

Circular RNA circ-PGAP3 promotes triple-negative breast cancer (TNBC) growth and metastasis by inhibiting miR-330-3p and upregulating Myc. This finding offers a potential therapeutic target for TNBC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) presents significant clinical challenges due to high recurrence rates, mortality, and limited treatment options.
  • Key factors driving TNBC progression remain incompletely understood, necessitating further research into novel molecular mechanisms.
  • Circular RNAs (circRNAs) are emerging as critical regulators in various cancers, including breast cancer.

Purpose of the Study:

  • To identify and characterize a novel circular RNA, circ-PGAP3, involved in triple-negative breast cancer.
  • To elucidate the biological functions and clinical significance of circ-PGAP3 in TNBC.
  • To investigate the underlying molecular mechanism by which circ-PGAP3 influences TNBC progression.

Main Methods:

  • Functional assays including CCK-8, colony formation, and Transwell invasion assays were performed.
  • RNA immunoprecipitation (RIP), RNA pull-down, and luciferase reporter assays were utilized to explore interactions between circ-PGAP3, miR-330-3p, and Myc.
  • In vivo experiments using an animal model were conducted to validate the role of circ-PGAP3.

Main Results:

  • Circ-PGAP3 expression is significantly elevated in TNBC tissues and correlates with advanced tumor stage, metastasis, and poor prognosis.
  • Circ-PGAP3 promotes TNBC cell growth and metastasis by acting as a sponge for miR-330-3p, leading to increased proto-oncogene Myc expression.
  • Experimental validation confirmed a positive correlation between circ-PGAP3 and Myc levels, and a negative correlation with miR-330-3p in TNBC tissues.

Conclusions:

  • Circ-PGAP3 plays a crucial role in driving the development and progression of triple-negative breast cancer.
  • The circ-PGAP3/miR-330-3p/Myc axis represents a significant pathway in TNBC pathogenesis.
  • Circ-PGAP3 emerges as a promising candidate therapeutic target for improving outcomes in TNBC patients.
Keywords:
Myccircular RNAmiRNAtriple negative breast cancer

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