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mRNA Stability and Gene Expression

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Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

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Published on: October 25, 2015

YTHDF2 Regulates Maternal Transcriptome Degradation and Embryo Development in Goat.

Mingtian Deng¹, BaoBao Chen², Zifei Liu¹

¹Jiangsu Livestock Embryo Engineering Laboratory, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Frontiers in Cell and Developmental Biology

|October 29, 2020

Summary

Maternal mRNA clearance is crucial for goat embryo development. The study found YTHDF2 protein is vital for clearing maternal mRNAs during zygotic genome activation, impacting early embryogenesis.

Keywords:

N6-methyladenosine YTHDF2 embryogenesis maternal mRNA decay maternal-to-zygotic transition zygotic genome activation

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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Area of Science:

Reproductive Biology
Molecular Embryology
Epigenetics

Background:

Maternal mRNA clearance is essential for early embryonic development.
RNA N6-methyladenosine (m⁶A) modification regulates maternal mRNA clearance.
Mechanisms of maternal mRNA clearance in farm animals, particularly goats, are poorly understood.

Purpose of the Study:

To identify maternal mRNAs during the maternal-to-zygotic transition (MZT) in goats.
To investigate the role of m⁶A reader YTHDF2 in maternal mRNA clearance and early goat embryogenesis.
To elucidate the mechanisms underlying ZGA-dependent maternal mRNA clearance.

Main Methods:

Differential gene expression analysis to identify maternal mRNAs in goat embryos.
YTHDF2 knockdown using RNA interference in goat embryos.
Assessment of embryonic development (blastocyst rate) and maternal mRNA levels in YTHDF2 knockdown embryos.
Analysis of deadenylase and decapping enzyme expression.

Main Results:

Identified 3362 differentially expressed genes as maternal mRNAs during goat MZT.
Found increased expression of m⁶A reader YTHDF2 during goat zygotic genome activation (ZGA).
YTHDF2 knockdown impaired maternal mRNA clearance (both M-decay and Z-decay) and reduced blastocyst rates, linked to decreased deadenylase and decapping enzyme expression.

Conclusions:

Maternal mRNA clearance in goats is dependent on ZGA.
YTHDF2 plays a critical role in goat early embryogenesis by promoting maternal mRNA clearance, potentially via recruitment of deadenylases and decapping enzymes.
This study provides insights into stochastic reprogramming during MZT and improving in vitro goat embryo development.