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Effects of cefmetazol, cefoxitin and imipenem on polymorphonuclear leukocytes
A B Rodriguez1, J Pariente, J Prieto
1Department of Animal Physiology, Faculty of Science, University of Extremadura, Badajoz, Spain.
Abstract:
1. We have investigated the effects produced in vitro by Cefmetazol, Cefoxitin and Imipenem on the chemotaxis, spontaneous mobility, adherence, phagocytosis and candidicide power of human polymorphonuclear neutrophils (PMNs). 2. The three antibiotics tested significantly stimulated the adherence of neutrophils to nylon fibre at doses either equal (50 mg/l) or superior (500 mg/l) to the therapeutic one. 3. Cefmetazol, Cefoxitin and Imipenem bring about a maximum increase of chemotaxis at the therapeutic dose, whereas the spontaneous mobility diminishes with any one of the doses used. 4. The capacity of the PMNs to phagocytize and produce lysis of Candida albicans is increased in Cefmetazol with therapeutic doses. Cefoxitin produced an increased lysis of candidas.
Insights
Three antibiotics, Cefmetazol, Cefoxitin, and Imipenem, enhance human neutrophil functions like adherence and chemotaxis. These immune cells also show improved ability to combat Candida albicans infections.
Area of Science:
- Immunology
- Pharmacology
Background:
- Neutrophils are critical immune cells involved in combating infections.
- Antibiotics can influence immune cell functions beyond their antimicrobial activity.
Purpose of the Study:
- To investigate the in vitro effects of Cefmetazol, Cefoxitin, and Imipenem on human polymorphonuclear neutrophil (PMN) functions.
- To assess the impact of these antibiotics on neutrophil chemotaxis, mobility, adherence, phagocytosis, and candidacidal activity.
Main Methods:
- In vitro analysis of human PMNs exposed to varying concentrations of Cefmetazol, Cefoxitin, and Imipenem.
- Evaluation of neutrophil adherence to nylon fiber.
- Assessment of neutrophil chemotaxis and spontaneous mobility.
- Measurement of phagocytosis and candidacidal power against Candida albicans.
Main Results:
- All three antibiotics significantly stimulated neutrophil adherence at therapeutic and supra-therapeutic doses.
- Cefmetazol, Cefoxitin, and Imipenem increased neutrophil chemotaxis at therapeutic doses.
- Spontaneous neutrophil mobility decreased across all tested doses.
- Cefmetazol enhanced PMN phagocytosis and lysis of Candida albicans, while Cefoxitin increased Candida lysis.
Conclusions:
- Cefmetazol, Cefoxitin, and Imipenem modulate human neutrophil functions in vitro, generally enhancing immune responses.
- These antibiotics may possess immunomodulatory properties that could influence their clinical efficacy, particularly in combating fungal infections.