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Joint Entropy-Assisted Graphene Oxide-Based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple
Youwen Zhang1, Xiaohan Chen1, Shaoqing Yuan2
1Department of Chemistry, Illinois Institute of Technology, 3101 South Dearborn Street, Chicago, Illinois 60616, United States.
Analytical Chemistry
|October 29, 2020
Summary
This study introduces a novel nanographene oxide (nGO) biosensor for simultaneously measuring multiple cancer biomarkers, matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinases (ADAMs). This multiplexing sensor offers a promising tool for early cancer detection and diagnosis.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Biochemistry
Background:
- Individual biomarkers have limited clinical utility for disease diagnosis and prognosis.
- Multiplex biomarker panels are increasingly recognized for improved accuracy and efficacy.
- Conventional sensor arrays for multiplex detection can be complex.
Purpose of the Study:
- To develop a non-array format multiplexing fluorescence sensor for simultaneous protease activity measurement.
- To utilize functionalized nanographene oxide (nGO) as a sensing element for cancer biomarkers.
- To demonstrate the application of joint entropy and programming for biomarker panel selection.
Main Methods:
- Development of a functionalized nanographene oxide (nGO) based biosensor.
- Utilized matrix metalloproteinases (MMPs) and a disintegrin and metalloproteinases (ADAMs) as model analytes.
- Simultaneous measurement of protease activities in simulated serum samples.
- Application of joint entropy and programming for guiding experiment design and biomarker panel selection.
Main Results:
- The nGO-based biosensor demonstrated rapid, sensitive, and selective detection of multiple proteases.
- Successful profiling of ADAMs/MMPs activities in simulated serum samples was achieved.
- Joint entropy and programming effectively guided the selection of an appropriate protease biomarker panel.
Conclusions:
- The developed nGO-based multiplex sensing platform enables simultaneous measurement of multiple protease activities.
- This platform shows significant potential for early cancer detection and diagnosis.
- The approach facilitates the rational design of biomarker panels for improved clinical utility.
