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Updated: Dec 3, 2025

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Phosphomatics: interactive interrogation of substrate-kinase networks in global phosphoproteomics datasets
Michael G Leeming1, Sean O'Callaghan2, Luana Licata3
1Melbourne Mass Spectrometry and Proteomics Facility, Bio21 Molecular Science & Biotechnology Institute, The University of Melbourne, Melbourne, VIC 3010, Australia.
Motivation:
Mass spectrometry-based phosphoproteomics can routinely identify and quantify thousands of phosphorylated peptides from a single experiment. However interrogating possible upstream kinases and identifying key literature for phosphorylation sites is laborious and time-consuming.
Results:
Here, we present Phosphomatics-a publicly available web resource for interrogating phosphoproteomics data. Phosphomatics allows researchers to upload phosphoproteomics data and interrogate possible relationships from a substrate-, kinase- or pathway-centric viewpoint.
Availability And Implementation:
Phosphomatics is freely available via the internet at: https://phosphomatics.com.
Supplementary Information:
Supplementary data are available at Bioinformatics online.
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