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The MemMoRF database for recognizing disordered protein regions interacting with cellular membranes.

Georgina Csizmadia1, Gábor Erdős2, Hedvig Tordai1

  • 1Department of Biophysics and Radiation Biology, Semmelweis University, Budapest 1094, Hungary.

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Intrinsically disordered protein regions (IDRs) interacting with lipids, termed MemMoRFs, are crucial for cellular processes. A new database centralizes validated MemMoRFs, aiding research into these dynamic protein regions.

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Protein-lipid interactions are vital for cellular functions like signaling and viral entry.
  • Intrinsically disordered protein regions (IDRs) can mediate these interactions, undergoing disorder-to-order transitions.
  • These lipid-interacting IDRs are termed Memorable lipid-induced disorder-to-order transitions (MemMoRFs).

Purpose of the Study:

  • To create a centralized, comprehensive database of experimentally validated MemMoRFs.
  • To facilitate the study and characterization of MemMoRFs in transmembrane and membrane-associated proteins.
  • To provide a user-friendly resource for researchers investigating these dynamic protein regions.

Main Methods:

  • Manual curation of literature and structural data to identify MemMoRFs.
  • Incorporation of secondary structure propensity and flexibility data from NMR chemical shifts.
  • Inclusion of functional and disease-related information alongside textual evidence from publications.

Main Results:

  • Generation of a comprehensive database of experimentally validated MemMoRFs.
  • Characterization of MemMoRF dynamics using NMR-derived flexibility and propensity data.
  • Integration of diverse data types, including literature, structure, function, and disease associations.

Conclusions:

  • The MemMoRF database serves as a central resource for studying disordered regions in membrane proteins.
  • This database aids in understanding the role of MemMoRFs in cellular processes and disease.
  • Improved accessibility and characterization of MemMoRFs will advance research in membrane biology.