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Updated: Dec 3, 2025

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
Published on: February 25, 2011
Intracellular Transport of Ribosome-Inactivating Proteins Depends on Annexin 13
D V Maltseva1,2,3, M P Raigorodskaya4, V G Zgoda5
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. dmaltseva@gmail.com.
Annexin 13 (ANXA13) protein impacts how cells transport type II ribosome-inactivating proteins (RIP-IIs). Reduced ANXA13 levels altered the cellular response to toxins like ricin and viscumin.
Area of Science:
- Cell Biology
- Molecular Biology
- Toxicology
Background:
- Annexin 13 (ANXA13) is a membrane-binding protein implicated in intracellular transport.
- Type II ribosome-inactivating proteins (RIP-IIs) are toxins that inhibit protein synthesis by modifying ribosomal RNA.
- Understanding the cellular mechanisms of RIP-IIs transport is crucial for developing countermeasures.
Purpose of the Study:
- To investigate the role of ANXA13 in the intracellular transport of vesicles containing RIP-IIs.
- To evaluate the impact of ANXA13 on the cytotoxic effects of specific RIP-II toxins.
Main Methods:
- Utilized a modified human intestinal epithelial cell line (HT29) with significantly reduced ANXA13 expression.
- Assessed the cytotoxic effects of ricin and viscumin toxins.
- Monitored toxin activity through modification of 28S ribosomal RNA.
Main Results:
- Differences in toxin activity were observed between parental and ANXA13-reduced HT29 cell lines.
- The findings suggest ANXA13 influences the intracellular transport pathway for RIP-II-containing vesicles.
- ANXA13 plays a distinct role in cellular response to specific RIP-II toxins.
Conclusions:
- ANXA13 is involved in the intracellular trafficking of vesicles carrying type II ribosome-inactivating proteins.
- Modulating ANXA13 expression affects cellular sensitivity to RIP-II toxins.
- This study provides insights into the cellular mechanisms governing toxin transport and cytotoxicity.
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