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Related Experiment Video

Updated: Dec 3, 2025

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
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Cognitive Enhancer Noopept Activates Transcription Factor HIF-1.

L F Zainullina1, T V Ivanova2, S V Sadovnikov2

  • 1Zakusov Research Institute of Pharmacology, Moscow, Russia.

Doklady. Biochemistry and Biophysics
|October 29, 2020
PubMed
Summary
This summary is machine-generated.

Noopept enhances the DNA-binding activity of hypoxia-inducible factor 1 (HIF-1) by stabilizing its HIF1α subunit. This involves inhibiting prolyl hydroxylase, impacting HIF-1-controlled gene expression.

Keywords:
FIHHIF-1HIF-1α stabilizationPro-Gly dipeptideshypoxianoopept

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Area of Science:

  • Molecular Biology
  • Neuroscience
  • Biochemistry

Background:

  • Hypoxia-inducible factor 1 (HIF-1) is a crucial transcription factor regulating cellular response to oxygen levels.
  • HIF-1 stabilization is critical for cellular adaptation to hypoxic conditions.
  • Understanding modulators of HIF-1 activity is important for therapeutic development.

Purpose of the Study:

  • To investigate the effect of Noopept on HIF-1 DNA-binding activity in vitro.
  • To elucidate the mechanisms by which Noopept stabilizes the HIF-1 transcription factor.
  • To examine the downstream effects of Noopept on HIF-1-regulated gene expression.

Main Methods:

  • In vitro studies using SH-SH5Y cells.
  • Assay of basal and induced HIF-1 DNA-binding activity.
  • Utilizing the ODD-Luc reporter assay to assess prolyl hydroxylase inhibition.
  • Analysis of HIF1α protein levels and gene expression changes.

Main Results:

  • Noopept significantly increased both basal and hypoxia-mimetic-induced HIF-1 DNA-binding activity.
  • Noopept was shown to inhibit HIF-1 prolyl hydroxylase, indirectly confirmed by ODD-Luc reporter data.
  • Noopept treatment led to an increased level of the HIF1α protein.
  • Gene expression analysis revealed changes in pathways controlled by HIF-1.

Conclusions:

  • Noopept enhances HIF-1 DNA-binding activity and stabilizes the HIF1α subunit.
  • The mechanism involves the inhibition of HIF-1 prolyl hydroxylase.
  • Noopept modulates gene expression related to various metabolic pathways regulated by HIF-1.