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Related Concept Videos

Patch Clamp01:18

Patch Clamp

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
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Clipper Circuit01:18

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A clipper circuit is a fundamental wave-shaping device that harnesses the unique properties of diodes to alter and control waveform characteristics. This technology is widely used in electronic devices, especially in television and radar communication systems, where it enhances waveform modulation in both transmitters and receivers.
The operation of a clipper circuit can be exemplified by analyzing a dual-clipper configuration setup that integrates two ideal diodes, each paired with a biasing...
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Clamper Circuit01:14

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A clamper circuit, also known as a DC restorer, represents a specialized variant of the rectifier circuit, notable for its method of taking the output across the diode rather than the capacitor. This configuration lends to several distinctive applications, particularly in handling square wave inputs.
Within this circuit, the diode's orientation prompts the capacitor to charge up to the level of the most negative peak of the input signal. Upon reaching this state, the diode ceases to...
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Updated: Dec 3, 2025

One-channel Cell-attached Patch-clamp Recording
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Patch Clamp Technology in the Twenty-First Century.

Jan Dolzer1

  • 1Sutter Instrument Company, Novato, CA, USA. jan@sutter.com.

Methods in Molecular Biology (Clifton, N.J.)
|October 29, 2020
PubMed
Summary

The patch clamp technique has evolved with new digital amplifier systems. Sutter Instrument

Area of Science:

  • Electrophysiology
  • Neuroscience
  • Biophysics

Background:

  • The patch clamp technique, a cornerstone in cellular electrophysiology, has seen significant evolution since its inception.
  • Instrumentation development for patch clamp systems experienced a slowdown in the mid-2000s after a surge in the 1990s.
  • Sutter Instrument's 2016 entry with turnkey patch clamp amplifier systems revitalized the field.

Purpose of the Study:

  • To compare the feature sets of Sutter Instrument's new digital patch clamp systems with established platforms.
  • To provide a comprehensive overview of patch clamp system architectures, headstage designs, and data acquisition strategies.
  • To discuss advanced technologies and application modules for patch clamp data analysis.

Main Methods:

  • Comparative analysis of Sutter Instrument's dPatch System against Axon Instruments and HEKA Elektronik platforms.
Keywords:
Data acquisitionDynamic clampIPAPatch clamp amplifierSingle-channel recordingSutterPatchWhole-celldPatch

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  • Review of architectural considerations, headstage design, and data management strategies for patch clamp systems.
  • Exploration of advanced features like digital signal compensation, dynamic clamp, and software lock-in amplifier capabilities.

    • Main Results:

    • Sutter Instrument's dPatch System offers a contemporary digital design with features like digital signal compensation, high bandwidth, and integrated dynamic clamp.
    • The comparison highlights the strengths of turnkey systems versus component-based approaches.
    • Advanced technologies and efficient data analysis modules are crucial for modern patch clamp applications.

    Conclusions:

    • The dPatch System represents a significant advancement in patch clamp instrumentation, offering innovative digital capabilities.
    • Continued innovation in patch clamp technology is essential for addressing complex research questions in neuroscience and beyond.
    • This work provides a valuable resource for researchers selecting and utilizing patch clamp systems.