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Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
Published on: January 23, 2014
Growth of retinal capillary endothelia using pericyte conditioned medium
H C Wong1, M Boulton, J Marshall
1Department of Clinical Ophthalmology, Institute of Ophthalmology, London, England.
Investigative Ophthalmology & Visual Science
|November 1, 1987
Abstract:
Long-term cultures of bovine retinal capillary endothelial cells (BRCEC) have been established using bovine retinal pericyte conditioned medium (PCM). PCM had greater mitogenic activity on BRCEC than unconditioned medium (P less than 0.01). The mitogenic activity of PCM was not cell specific since bovine Tenon's fibroblast conditioned medium also had significant activity. PCM's activity was not enhanced by heparin though it was additive to that of retinal crude extract.
