Preparation, characterization and wound healing effect of vaccarin-chitosan nanoparticles

Wound healing is a complex, dynamic and difficult process. Much effort and attempt has been made to accelerate this process. The purpose of this study is to prepare nanoparticles loaded with vaccarin (VAC-NPS)hydrogel and evaluate its effect on promoting wound healing. In the present study, the physicochemical properties of VAC-NPS were characterized. Transmission electron microscopy (TEM) was used to observe the morphology of VAC-NPS. Human umbilical vein endothelial cells (HUVEC) was employed to assessment the biocompatibility of VAC-NPS in vitro. The wound healing function of VAC-NPS hydrogels was evaluated in the full-thickness dermal wound in a rat model. The results indicated that VAC-NPS was spherical like particles with uniform particle size distribution and no obvious aggregation with a diameter of (216.6 ± 10.1)nm. The loading capacity and encapsulation efficiency of VAC in the nanoparticles were (14.3 ± 1.2) % and (51.7 ± 1.7) % respectively. MTT assay demonstrated that the VAC-NPS had no cytotoxicity and could promote HUVEC proliferation and migration. In vivo results showed that VAC-NPS promotes wound healing, and the mechanism may be through up-regulating IL-1β and PDGF-BB, promoting angiogenesis. VAC-NPS might have a potential application value for the treatment of the wound healing and a promising performance in bio-medically relevant systems.