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miR-142-5p promotes renal cell tumorigenesis by targeting TFAP2B

Maoshu Zhu1, Liangneng Zou2, Fuhua Lu3

  • 1The Central Laboratory, The Fifth Hospital of Xiamen, Xiamen, Fujian 361101, P.R. China.

Oncology Letters
|October 30, 2020
PubMed

Insights

MicroRNA-142-5p targets TFAP2B, promoting clear cell renal cell carcinoma (ccRCC) cell growth and migration. This suggests miR-142-5p acts as an oncogene in ccRCC development.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Transcription factor AP-2 beta (TFAP2B) is crucial for kidney development.
  • MicroRNAs (miRNAs) are implicated in cancer, including clear cell renal cell carcinoma (ccRCC).
  • The regulatory relationship between miRNAs and TFAP2B in ccRCC remains unclear.

Purpose of the Study:

  • To investigate the effect of miRNAs on TFAP2B expression.
  • To explore the role of miRNA-TFAP2B interaction in ccRCC cell proliferation, invasion, and migration.

Main Methods:

  • Polymerase Chain Reaction (PCR) and Western Blotting to assess TFAP2B expression.
  • Dual Luciferase Reporter Assays to confirm miRNA targeting.
  • MTT assays, Flow Cytometry, Wound Healing, and Transwell assays to evaluate cell behavior.

Main Results:

  • miR-142-5p was identified as a regulator of TFAP2B.
  • miR-142-5p significantly downregulated TFAP2B expression at both mRNA and protein levels.
  • miR-142-5p promoted proliferation and migration in ccRCC cell lines (786-O and A-498).

Conclusions:

  • miR-142-5p targets TFAP2B and promotes ccRCC progression.
  • miR-142-5p may function as an oncogene in ccRCC.
  • miR-142-5p holds potential as a biomarker and therapeutic target for ccRCC.
Keywords:
miR-142-5pmigrationproliferationrenal cell carcinomatranscription factor AP-2 β

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