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Identification of AK4 as a novel therapeutic target for serous ovarian cancer
Minmin Huang1, Xinlei Qin1, Yuwei Wang1
1Department of Gynaecology and Obstetrics, The Second People's Hospital of Lianyungang, Lianyungang, Jiangsu 222023, P.R. China.
Abstract:
The present study aimed to assess the expression level of adenylate kinase 4 (AK4) in human serous ovarian cancer (SOC) tissues and investigate the possible involvement of AK4 in SOC progression. Bioinformatics analysis based on The Cancer Genome Atlas (TCGA) database and immunohistochemical (IHC) assays were performed to assess the expression level of AK4 in human SOC tissues. Clinical pathological features of patients with SOC were also evaluated. Colony formation, MTT, wound healing and Transwell assays were conducted to investigate the effects of AK4 on the proliferation, migration, and invasion of SOC cells in vitro. Mouse xenograft and lung metastasis models were developed to evaluate the effects of AK4 on tumor growth and metastasis in vivo. High expression levels of AK4 were identified in human SOC tissues compared with in normal tissues according to TCGA database and the results of IHC assays. A contribution of AK4 to tumor growth and metastasis of SOC cells in vivo was also shown. The present study confirmed the involvement of AK4 in the progression of SOC, and the results indicated that AK4 could serve as a novel therapeutic target for SOC treatment.
Insights
Adenylate kinase 4 (AK4) is highly expressed in serous ovarian cancer (SOC), driving tumor growth and metastasis. Targeting AK4 presents a potential new therapeutic strategy for ovarian cancer patients.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Serous ovarian cancer (SOC) remains a leading cause of cancer-related mortality.
- Understanding the molecular mechanisms driving SOC progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the expression of adenylate kinase 4 (AK4) in human SOC tissues.
- To explore the role of AK4 in the proliferation, migration, invasion, and metastasis of SOC cells.
- To evaluate AK4 as a potential therapeutic target for SOC.
Main Methods:
- Bioinformatics analysis using The Cancer Genome Atlas (TCGA) database.
- Immunohistochemistry (IHC) assays on human SOC tissues.
- In vitro assays (colony formation, MTT, wound healing, Transwell) to assess cell behavior.
- In vivo studies using mouse xenograft and lung metastasis models.
Main Results:
- Elevated AK4 expression was observed in SOC tissues compared to normal tissues.
- AK4 significantly promoted the proliferation, migration, and invasion of SOC cells in vitro.
- AK4 enhanced tumor growth and metastasis in vivo mouse models.
Conclusions:
- AK4 plays a significant role in the progression of serous ovarian cancer.
- The findings suggest that AK4 is a potential novel therapeutic target for SOC treatment.
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