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Efficacy of low-dose local clindamycin in different times for microbial decontamination of autogenous particulate
Hassan Mohajerani1, Gholamreza Irajian2, Fatemeh Latifi3
1Department of Oral and Maxillofacial Surgery, Shahid Beheshti University of Medical Sciences, Velenjak, Tehran, Iran.
Background:
Clindamycin in low concentration (20 μg/mL) is safe for vitality and osteogenic potential of bone cells. The aim of this study was to evaluate the efficacy of local clindamycin (20 μg/mL) in two different exposure times, for microbial decontamination of particulate bone graft, collected during implant site preparation. This non-randomized parallel-group study was conducted on samples from 17 patients. The particulate bone collected during implant site preparation was divided into three portions by weight: in group S1, the particulate bone was immersed in thioglycolate broth without any antibiotic treatment; in group S2, the collected particulate bone was irrigated with 100 mL clindamycin solution (20 μg/mL); and in group S3, the collected particulate bone was soaked in one ml clindamycin solution (20 μg/mL) for 3 min. Samples in the three groups were cultured in aerobic and anaerobic media and species and CFU count of isolated bacteria were determined.
Results:
Analysis of the data demonstrated a significant difference among the three groups in the mean count of total microorganisms (P = 0.001). The difference in the mean count of anaerobic and aerobic microorganisms in the three groups was statistically significant as well (P = 0.001). Pseudomonas aeruginosa was the only microorganism that was not affected with the mentioned antibiotic.
Conclusions:
Local use of low-dose clindamycin (20 μg/mL)-irrigation or 3 min immersing-is effective for the decontamination of particulate bone grafts.
