Survival of Mycobacterium paratuberculosis in slurry
Nordisk Veterinaermedicin
|June 1, 1977
Abstract:
Cattle and swine slurry and a mixture of equal parts of both, was mixed with a culture of M. paratuberculosis, 0.1 mg per ml (1 mg = 33 X 10(6) viable units) and stored under anaerobic conditions at 5 degrees and 15 degrees C. At 5 degrees C the survival time for M. paratuberculosis was 252 days in all 3 kinds of slurry, and at 15 degrees C it was 98 days in cattle slurry, 182 days in swine slurry, and 168 days in mixed slurry.
Insights
Mycobacterium avium subspecies paratuberculosis (MAP) survived for extended periods in cattle and swine slurry stored anaerobically. Survival was longer at 5°C (252 days) than at 15°C, with variations across slurry types.
Area of Science:
- Environmental microbiology
- Veterinary science
- Bacteriology
Background:
- Mycobacterium avium subspecies paratuberculosis (MAP) is an important veterinary pathogen.
- Understanding MAP survival in animal waste is crucial for disease control and environmental management.
Purpose of the Study:
- To determine the survival time of MAP in cattle slurry, swine slurry, and a mixture of both.
- To assess the impact of storage temperature (5°C and 15°C) on MAP survival in slurry.
Main Methods:
- MAP cultures were added to cattle slurry, swine slurry, and a 50:50 mixture.
- Samples were stored under anaerobic conditions at 5°C and 15°C.
- MAP survival was monitored over time.
Main Results:
- At 5°C, MAP survived for 252 days in all three slurry types.
- At 15°C, MAP survival was 98 days in cattle slurry, 182 days in swine slurry, and 168 days in mixed slurry.
Conclusions:
- MAP can persist in cattle and swine slurry for significant periods, especially at lower temperatures.
- Environmental conditions, including temperature and slurry composition, influence MAP viability.
- These findings have implications for manure management and the potential spread of paratuberculosis.
